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Abstract Objectives: Evaluation of the repeatability of occlusal registration performed with the T-scan in the same individual on two different days. Methods: A calibrated operator performed 46 measurements on 23 volunteers, with a time interval of seven days using the T-scan (v.9). Maximum intercuspation and maximum force values were assessed by tooth and individual. The repeatability of the device was determined by the mean of the intraclass correlation coefficient. The results were presented as mean +/- 95% confidence interval, and the data analysis was performed using SPSS software. Results: Significant differences were found in teeth 12 and 11 in maximum force and teeth 13, 12, and 11 in maximum intercuspation (p<0.05). The two measurements performed showed a high intraclass correlation coefficient value, both for maximum intercuspation and for maximum force (99.5% [99.4, 99.6] and 97.9% [97.5, 98.2]), respectively. Conclusions: The results obtained in this study suggest that the T-scan can be considered a complementary means for occlusal diagnosis and that these measurements show high intra-individual repeatability over time.

Resumo Objetivos: Avaliar a repetibilidade intra-indivíduo do registo oclusal realizado com o T-scan em dois momentos temporais. Métodos: Foram avaliados 23 voluntários com 46 medições com o T-scan (v.9) por um operador calibrado, com um intervalo de tempo de sete dias. Avaliaram-se os valores em intercuspidação e força máxima total e por dente, em cada indivíduo. A repetibilidade do aparelho foi determinada através do coeficiente de correlação intraclasse. Os resultados foram apresentados como média +/- intervalo de confiança a 95% e a sua análise realizada com recurso ao software SPSS. Resultados: Foram detetadas diferenças significativas entre medições nos dentes 12 e 11 em forç,a máxima de mordida e 13, 12 e 11 em intercuspidação máxima entre a primeira e segunda avaliações (p<0,05). Os dois momentos temporais apresentaram um coeficiente de correlação intraclasse elevado tanto para a intercuspidação máxima como para a força máxima de mordida, com valores de 99,5% [99,4; 99,6] e 97,9% [97,5; 98,2], respetivamente. Conclusões: Os resultados obtidos neste estudo sugerem que o T-scan poderá ser considerado um meio complementar de diagnóstico oclusal e que as medições apresentam uma elevada repetibilidade intra-indivíduo.

https://doi.org/10.24873/j.rpemd.2023.07.1057

O que mudou entre os períodos de pico e de platô durante a primeira onda do SARS-CoV-2? Estudo multicêntrico português em unidades de cuidados intensivos
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Pereira, Rui Antunes

; Sousa, Marta

; Cidade, José Pedro

; Melo, Luís

; Lopes, Diogo

; Ventura, Sara

; Aragão, Irene

; Lima Neto, Raul Miguel de Freitas

; Molinos, Elena

; Marques, Ana

; Cardoso, Nelson

; Marino, Flávio

; Monteiro, Filipa Brás

; Oliveira, Ana Pinho

; Silva, Rogério C

; Real, André Miguel Neto

; Banheiro, Bruno Sarmento

; Reis, Renato

; Adão-Serrano, Maria

; Cracium, Ana

; Valadas, Ana

; Ribeiro, João Miguel

; Póvoa, Pedro

; Tapadinhas, Camila

; Mendes, Vítor

; Coelho, Luís

; Maia, Raquel

; Freitas, Paulo Telles

; Ferreira, Isabel Amorim

; Ramires, Tiago

; Val-Flores, Luís Silva

; Cascão, Mariana

; Alves, Rita

; Rodeia, Simão C

; Barrigoto, Cleide

; Cardiga, Rosa

; Silva, Maria João Ferreira da

; Vale, Bruno

; Fonseca, Tatiana

; Rios, Ana Lúcia

; Camões, João

; Pérez, Danay

; Cabral, Susana

; Ribeiro, Maria Inês

; Mendes, João João

; Gouveia, João

; Fernandes, Susana Mendes

Revista Brasileira de Terapia Intensiva

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Dez 2022, Volume 34 Nº 4 Páginas 433 - 442

Resumo: En Pt | Texto: En Pt | PDF: En | PDF: Pt

ABSTRACT Objective: To analyze and compare COVID-19 patient characteristics, clinical management and outcomes between the peak and plateau periods of the first pandemic wave in Portugal. Methods: This was a multicentric ambispective cohort study including consecutive severe COVID-19 patients between March and August 2020 from 16 Portuguese intensive care units. The peak and plateau periods, respectively, weeks 10 - 16 and 17 - 34, were defined. Results: Five hundred forty-one adult patients with a median age of 65 [57 - 74] years, mostly male (71.2%), were included. There were no significant differences in median age (p = 0.3), Simplified Acute Physiology Score II (40 versus 39; p = 0.8), partial arterial oxygen pressure/fraction of inspired oxygen ratio (139 versus 136; p = 0.6), antibiotic therapy (57% versus 64%; p = 0.2) at admission, or 28-day mortality (24.4% versus 22.8%; p = 0.7) between the peak and plateau periods. During the peak period, patients had fewer comorbidities (1 [0 - 3] versus 2 [0 - 5]; p = 0.002) and presented a higher use of vasopressors (47% versus 36%; p < 0.001) and invasive mechanical ventilation (58.1 versus 49.2%; p < 0.001) at admission, prone positioning (45% versus 36%; p = 0.04), and hydroxychloroquine (59% versus 10%; p < 0.001) and lopinavir/ritonavir (41% versus 10%; p < 0.001) prescriptions. However, a greater use of high-flow nasal cannulas (5% versus 16%, p < 0.001) on admission, remdesivir (0.3% versus 15%; p < 0.001) and corticosteroid (29% versus 52%, p < 0.001) therapy, and a shorter ICU length of stay (12 days versus 8, p < 0.001) were observed during the plateau. Conclusion: There were significant changes in patient comorbidities, intensive care unit therapies and length of stay between the peak and plateau periods of the first COVID-19 wave.

RESUMO Objetivo: Analisar e comparar as características de pacientes críticos com a COVID-19, a abordagem clínica e os resultados entre os períodos de pico e de platô na primeira onda pandêmica em Portugal. Métodos: Este foi um estudo de coorte multicêntrico ambispectivo, que incluiu pacientes consecutivos com a forma grave da COVID-19 entre março e agosto de 2020 de 16 unidades de terapia intensiva portuguesas. Definiram-se as semanas 10 - 16 e 17 - 34 como os períodos de pico e platô. Resultados: Incluíram-se 541 pacientes adultos com mediana de idade de 65 [57 - 74] anos, a maioria do sexo masculino (71,2%). Não houve diferenças significativas na mediana de idade (p = 0,3), no Simplified Acute Physiology Score II (40 versus 39; p = 0,8), na pressão parcial de oxigênio/fração inspirada de oxigênio (139 versus 136; p = 0,6), na terapia com antibióticos na admissão (57% versus 64%; p = 0,2) ou na mortalidade aos 28 dias (24,4% versus 22,8%; p = 0,7) entre o período de pico e platô. Durante o período de pico, os pacientes tiveram menos comorbidades (1 [0 - 3] versus 2 [0 - 5]; p = 0,002); fizeram mais uso de vasopressores (47% versus 36%; p < 0,001) e ventilação mecânica invasiva na admissão (58,1% versus 49,2%; p < 0,001), e tiveram mais prescrição de hidroxicloroquina (59% versus 10%; p < 0,001), lopinavir/ritonavir (41% versus 10%; p < 0,001) e posição prona (45% versus 36%; p = 0,04). Entretanto, durante o platô, observou-se maior uso de cânulas nasais de alto fluxo (5% versus 16%; p < 0,001) na admissão, remdesivir (0,3% versus 15%; p < 0,001) e corticosteroides (29% versus 52%; p < 0,001), além de menor tempo de internação na unidade de terapia intensiva (12 versus 8 dias; p < 0,001). Conclusão: Houve mudanças significativas nas comorbidades dos pacientes, nos tratamentos da unidade de terapia intensiva e no tempo de internação entre os períodos de pico e platô na primeira onda da COVID-19.

https://doi.org/10.5935/0103-507x.20210037-pt

CO2 capture and water use efficiency in Opuntia stricta (Haw.) at different seasons and evaluation times
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SOUZA, JOSÉ THYAGO AIRES

; ARAÚJO, JUCILENE SILVA

; FÉLIX, EVALDO DOS SANTOS

; ALVES, RITA DE CÁSSIA

; FILHO, TARCÍSIO JOSÉ DE OLIVEIRA

; LIRA, ELDER CUNHA DE

Revista Colombiana de Ciencias Hortícolas

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Mai 2022, Volume 16 Nº 2 elocation e13525

Resumo: Es En | Texto: Es En | PDF: Es | PDF: En

RESUMEN El nopal forrajero Oreja de Elefante Mexicano está ampliamente incorporado a la cadena productiva animal de la región noreste de Brasil. Sin embargo, faltan estudios sobre la dinámica fisiológica de este cactus. Por lo anterior, se realizó este estudio en la Estação Experimental Prof. Ignácio Salcedo del Instituto Nacional do Semiárido (INSA), en Campina Grande, estado de Paraíba, Brasil. El objetivo del estudio fue evaluar la absorción de CO2 y los niveles de eficiencia en el uso del agua in Opuntia stricta (Haw.) durante diferentes estaciones e horas de evaluación. Los tratamientos se distribuyeron utilizando un arreglo factorial de 24×2, que correspondió a la evaluación de los procesos de intercambio de gases durante 24 horas en la época de lluvias (junio) y sequía (diciembre). Los parámetros evaluados fueron la conductancia estomática, la tasa de transpiración y absorción de CO2, la concentración interna de CO2, la eficiencia instantánea de uso del agua y la eficiencia intrínseca de uso del agua. Los resultados revelaron que el intercambio de gases en el nopal forrajero son más intensos durante la estación de lluvias, con buena estabilidad, que los bajos niveles de intercambio durante la estación época de sequía. Independientemente de la estación, la absorción de CO2 alcanzó su pico entre las 24:00 y las 2:00. Además, este intervalo de tiempo es el más adecuado para realizar análisis en condiciones de campo.

ABSTRACT The forage cactus Mexican Elephant Ear is widely incorporated into the animal productive chain of the northeast region of Brazil. However, there is a lack of studies on the physiological dynamics of this cactus. Therefore, this study was conducted at the Estação Experimental Prof. Ignácio Salcedo of the Instituto Nacional do Semiárido (INSA), in Campina Grande, State of Paraíba, Brazil. The aim of this study was to evaluate CO2 uptake and water-use efficiency levels in Opuntia stricta (Haw.) during different seasons and evaluation times. The treatments were distributed using a 24×2 factorial arrangement, which corresponded to the evaluation of gas exchange processes for 24 hours in the rainy (June) and dry (December) seasons. The evaluated parameters were stomatal conductance, transpiration and CO2 uptake rate, internal CO2 concentration,instantaneous water-use efficiency and intrinsic water-use efficiency. The results revealed that gas exchange in the forage cactus was more intense during the rainy season, with good stability, than the low exchange levels during the dry season. Regardless of the season, the CO2 uptake peaked between 24:00 and 02:00. In addition, this range of time is the most suitable to conduct analyses under field conditions.

https://doi.org/10.17584/rcch.2022v16i2.13525

Classification of homogeneous regions of vegetation cover in the State of Rio Grande do Sul, Brazil and its temporal dynamics, using AVHRR GIMMS and MODIS data sets
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CORDEIRO, ANA PAULA A.

; ALVES, RITA DE CÁSSIA M.

; STEFFLER, ANA PAULA L.W.

; MENGUE, VAGNER P.

; FONTANA, DENISE C.

; ROGLIO, VINICIUS S.

; GUASSELLI, LAURINDO A.

Anais da Academia Brasileira de Ciências

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2021, Volume 93 Nº 3 elocation e20201278

Resumo: En | Texto: En | PDF: En

Abstract This study aimed to classify the homogeneous regions of vegetation cover, which occur in Rio Grande do Sul, formed by clustering of pixels with same pattern of temporal variability of the Normalized Difference Vegetation Index (NDVI) of AVHRR GIMMS and MODIS series and to compare their temporal dynamics. We use K means cluster analysis for defining homogeneous regions, based on the temporal variability of GIMMS (8 km spatial resolution) and MODIS (1 km spatial resolution) NDVI data sets, using monthly images mean from 2000 to 2008 (overlapping period); and we analyzed the annual pattern of NDVI. Accuracy assessment was done with Landsat images. The results show that the temporal variability of GIMMS and MODIS NDVI allows to delimit similar homogeneous regions in order to mapping the main vegetation cover. MODIS series shows a greater detail in the definition of the regions, but with compatibility with those generated by GIMMS. The temporal dynamics show a typical seasonal pattern, with variations of NDVI amplitude between the groups, that allow to monitor phenological changes. The deviations from calibration between times series are linear, which would facilitate a correction in order to construct a long synthetic time series for studies of land cover change.

https://doi.org/10.1590/0001-3765202120201278 134 downloads

Comparing explosive cyclogenesis cases of different intensities occurred in Southern Atlantic
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AVILA, VILSON D. DE

; NUNES, ANDRÉ B.

; ALVES, RITA DE CÁSSIA M.

Anais da Academia Brasileira de Ciências

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2021, Volume 93 Nº 3 elocation e20190157

Resumo: En | Texto: En | PDF: En

Abstract Six events of explosive cyclogenesis occurred in the south Atlantic were compared using reanalysis data and satellite water vapor imagery. Cases of different intensities (weak, moderate and strong) occurred during 2014 summer season and 2012 winter season were studied. Despite the similarities the tropopause anomaly was more prominent and vertical movements were stronger in the strong cyclogenesis cases. The tropopause anomalies behind the cold front and ahead of the warm front appear only in the mature stage of the weak and moderate cases while in the strong case it is already evident and more intense behind the cold front since the beginning of the cycle. In all the cases confluence of the jet streams took place at higher levels forming a jet streak with difluence occurring downstream and the cyclone beginning in the exit region. The trajectories of the cyclones were in the southeast direction but longer and more meridional in the strong cases. The results indicated the baroclinicity of the region as the main mechanism for the development of these cyclones as well as the amplitude of the upper level jet stream perturbation. Furthermore, all the explosive cyclones developed following the Shapiro & Keyser cyclone conceptual model.

https://doi.org/10.1590/0001-3765202120190157 116 downloads

Asymmetric parasitic twins - Heteropagus
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Alves, Rita de Cássia S.

; Petry, Adriana Ubirajara Silva

; Marçal, Josenel Maria Barcelos

; Hochhegger, Bruno

; Damin, Andréa Souto

; Roehe, Adriana Vial

Revista da Associação Médica Brasileira

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Nov 2020, Volume 66 Nº 11 Páginas 1526 - 1529

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

RESUMO BACKGROUND: Gêmeos parasitas assimétricos ou heterópagos são uma ocorrência rara, com incidência de um caso em 1-2 milhões. Os gêmeos siameses são classificados de acordo com sua simetria, local de fusão e grau de duplicação. MÉTODOS: Relatamos aqui uma apresentação extremamente rara de gêmeos parasitários não descritos anteriormente. São descritas alterações macro e microscópicas e discutidos aspectos relevantes dessa malformação e da importância do diagnóstico pré-natal. RESULTADOS: Um caso de uma paciente de 23 anos de idade, com gestação monocoriônica, monoamniótica de gêmeos siameses assimétricos diagnosticada com 29 semanas de idade gestacional. Acreditamos que este relato chama a atenção para esta apresentação e para a importância do cuidado e manejo pré-natal. Os fetos nasceram de parto vaginal já sem vida, pesando em conjunto 1.300 gramas, e eram unidos pela região cervical esquerda. CONCLUSÃO: Este relato pode ajudar a encontrar estratégias para a decisão clínica em casos futuros. O diagnóstico pré-natal é fundamental para o manejo e planejamento pré-operatório. Exames de imagem como ecocardiografia, tomografia computadorizada, ressonância magnética e ultrassonografia são factíveis e podem fornecer informações-chave sobre a gravidade e prognóstico da malformação.

SUMMARY BACKGROUND: Asymmetric or heteropagus conjoined twins is a rare occurrence, with an incidence of one case in 1-2 million. Conjoined twins are classified according to their symmetry, place of fusion, and grade of duplication. METHODS: We report here an extremely rare presentation of parasitic twins not described before. We describe macro and micro anatomic alterations and discuss the aspects of this peculiar presentation and the importance of prenatal diagnosis. RESULTS: The case of a twenty-three-year-old patient, with monochorionic, monoamniotic asymmetrically-conjoined twin pregnancy, discovered at 29 weeks of gestational age. We believe that this report calls attention to this presentation and the importance of prenatal care and management. The twins were delivered vaginally without life. The twins’ combined weight was 1.300 gr. They were bonded in the left cervical region. CONCLUSION: This report may help to find strategies for clinical decisions in future cases. Antepartum diagnosis is important to the management, preoperative planning, and outcomes. Prenatal imaging exams like echocardiography, CT, MRI, and ultrasonography are feasible and can provide relevant information about malformation severity and prognosis.

https://doi.org/10.1590/1806-9282.66.11.1526 564 downloads

Seasonal variation of vitamin D among healthy adult men in a subtropical region
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Fontanive, Tiago Oselame

; Dick, Nidea Rita Michels

; Valente, Mariana Costa Silva

; Laranjeira, Vani dos Santos

; Antunes, Marina Venzon

; Corrêa, Marcelo de Paula

; Alves, Rita de Cássia Marques

; Linden, Rafael

; Furlanetto, Tania Weber

Revista da Associação Médica Brasileira

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Out 2020, Volume 66 Nº 10 Páginas 1431 - 1436

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

RESUMO OBJETIVOS: Avaliar a sazonalidade da 25(OH)vitamina D3 [25(OH)D3] e fatores associados em homens adultos saudáveis, que se exercitavam ao ar livre pelo menos 50 min duas vezes por semana, das 10 às 16h, em uma região subtropical. MÉTODOS: Sangue foi colhido no fim das estações para medir 25(OH)D3, por cromatografia líquida em tandem com espectroscopia de massas. A radiação ultravioleta foi estimada por radiômetro, calculando diariamente a resposta fotobiológica para sintetizar vitamina D na pele humana (D-VitD). RESULTADOS: A prevalência de 25(OH)D3 <20ng/mL foi sazonal (p=0.000): 8.7% (n=6/69), 1.5% (n=1/66), 0% (n= 0/64), e 21.7% (n=13/60), respectivamente, no final do inverno, primavera, verão e outono. A prevalência, ajustada para comparações múltiplas, foi maior no inverno do que no verão (p=0.026) e no outono do que na primavera (p=0.001) e verão (p=0.000). A 25(OH)D3 não se associou com o índice de massa corporal (p=0.207), gordura corporal (p=0.064) ou fototipo (p=0.485), na análise univariada. Associou-se à D-VitD nos 30 dias antes da coleta de sangue (p=0.000), ajustada para gordura corporal. Houve sazonalidade na prevalência de 25(OH)D3 <30ng/mL (p=0.000): 69.6% (n=48/69), 68.2% (n=45/66), 43.8% (n=28/64), e 88.4% (n=53/60), respectivamente, no inverno, primavera, verão e outono. CONCLUSÕES: Em uma região subtropical, houve sazonalidade na 25(OH)D3 em homens adultos, saudáveis, embora se exercitassem ao ar livre pelo menos 50 minutos duas vezes por semana, usando shorts e camiseta. 25(OH)D3 <20ng/mL foi 21.7% no outono e a D-vitD 30 dias antes da coleta do sangue foi o único fator associado de modo independente à 25(OH)D3.

SUMMARY OBJECTIVE: To evaluate seasonal variation of 25(OH)vitamin D [25(OH)D3] levels, and factors associated with it, in healthy adult men, who exercised outdoors for 50 min., at least twice a week, from 10AM to 4PM, in a Brazilian semitropical region. METHODS: Blood samples were collected at the end of each season for 25(OH)D3, measured by liquid chromatography with tandem mass spectrometry. Ultraviolet irradiation was estimated by radiometer, calculating the daily photobiological response to vitamin D synthesis in human skin (D-VitD). The prevalence of 25(OH)D3 <20ng/mL changed with the seasons (p=0.000): 8.7% (n=6/69), 1.5% (n=1/66), 0 (n=0/64), and 21.7% (n=13/60), respectively, at the end of winter, spring, summer, and autumn. The prevalence, adjusted for multiple comparisons, was higher in winter than summer (p=0.026), and in autumn than spring (p=0.001) and summer (p=0.000). There were no associations of 25(OH) D3 levels with BMI (p=0.207), body fat (p=0.064), and phototype (p=0.485), in univariate analysis. It was associated with D-VitD in the 30 days before blood sampling (p=0.000), after adjustment to body fat. The prevalence of 25(OH)D3 <30ng/mL varied seasonally (p=0.000): 69.6% (n=48/69), 68.2% (n=45/66), 43.8% (n=28/64), and 88.4% (n=53/60), respectively, in winter, spring, summer, and autumn. CONCLUSIONS: In a Brazilian subtropical region, a seasonal variation in 25(OH)D3 was observed in healthy adult males, although they spent at least 50 min outdoors twice a week, wearing shorts and T-shirts. 25(OH)D3 <20ng/mL was 21.7% in autumn; D-vitD 30 days prior to blood sampling was the only factor independently associated with 25(OH)D3 levels.

https://doi.org/10.1590/1806-9282.66.10.1431 612 downloads

Simultaneous pancreas-kidney transplantation after liver transplantation: first Portuguese case report
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Soares, Elsa

; Alves, Rita Valério

; Cotovio, Patrícia

; Caeiro, Fernando

; Aires, Inês

; Bigotte, Miguel

; Silva, Cecília

; Pena, Ana

; Bicho, Luís

; Martins, Américo

; Remédio, Francisco

; Ferreira, Aníbal

; Nolasco, Fernando

Portuguese Journal of Nephrology & Hypertension

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Set 2020, Volume 34 Nº 3 Páginas 174 - 176

Resumo: En | Texto: En | PDF: En

Simultaneous kidney pancreas transplantation, if feasible, is the treatment of choice for the management of end-stage renal disease in patients with type 1 diabetes. This intervention can offer glycemic control and might also stabilize or even reverse some of the metabolic complications of diabetes. Chronic kidney disease has become a critical problem in orthotopic liver transplantation due to multiple risk factors, including post-transplant diabetes mellitus and immunosuppression. This has a major impact on graft and patient survival. In this paper we present the case of a 42-year-old man submitted to a simultaneous pancreas kidney transplant after a successful liver transplantation 18 years earlier. The surgical procedure was uneventful, and the patient was discharged 18 days after admission. The case findings support that simultaneous pancreas and kidney transplantation is an effective method for the treatment of chronic kidney disease in patients with diabetes and a previous liver transplant.

https://doi.org/10.32932/pjnh.2020.10.090 346 downloads

Neutropenic enterocolitis (typhlitis) in a kidney transplant patient: A case report
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Alves, Rita Valério

; Falcão, Luís

; Soares, Elsa

; Mendes, Milena

; Cotovio, Patrícia

; Caeiro, Fernando

; Aires, Inês

; Silva, Cecília

; Remédio, Francisco

; Ferreira, Aníbal

; Nolasco, Fernando

Portuguese Journal of Nephrology & Hypertension

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Jun 2020, Volume 34 Nº 2 Páginas 116 - 118

Resumo: En | Texto: En | PDF: En

Typhlitis is a clinical entity rare in adults, reported in immunosuppressed patients. It is a life-threatening condition that occurs in neutropenic patients due to compromised integrity of the bowel wall and its exact pathogenesis is not completely understood. We report a 35-year-old man with a post deceased donor renal and pancreas transplantation status who presented fever and febrile neutropenia. Three days after admission he presented hematochezia with a hemoglobin drop. Colonoscopy revealed "ileum mucosa with multiforme ulcers" and since no other etiology was found, typhlitis was considered. He gradually improved with support treatment and was able to be managed non-operatively. To our knowledge, there are only six other reports of typhlitis in kidney transplant recipients.

https://doi.org/10.32932/pjnh.2020.07.077 358 downloads

10.

Salt stress alleviation by seed priming with silicon in lettuce seedlings: an approach based on enhancing antioxidant responses
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Alves, Rita de Cássia

; Nicolau, Mayara Cristina Malvas

; Checchio, Mirela Vantini

; Sousa Junior, Gilmar da Silveira

; Oliveira, Francisco de Assis de

; Prado, Renato Melo

; Gratão, Priscila Lupino

Bragantia

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Mar 2020, Volume 79 Nº 1 Páginas 19 - 29

Resumo: En | Texto: En | PDF: En

ABSTRACT Seed germination and seedling growth are the stages most sensitive to salt stress, which can induce ion-specific effects and oxidative stress. Although silicon (Si) has been known to improve plant defense systems against stressful-conditions, little is known about seed priming with Si and mechanisms of Si-mediated alleviation of salt stress in seedlings. To further the modulation of salt-stress responses and their relation with Si, seed priming with calcium silicate (0, 0.05 and 0.1 mM Ca2SiO4) was used in lettuce seeds to improve seedgermination and tolerance on subsequent salt-stress exposure (0 and 50 mM NaCl). The effect of Si-priming was assessed on germination, germination rate index and mean germination time and survival of seedlings in salt-stressful conditions supported by biochemical approach involving antioxidant responses. Overall results indicated that Si plays a role in alleviating the negative effects of salt stress by improving germination performance and displayed increased SOD, CAT and GR activities and reduced malondialdehyde and H2O2 contents. Thus, our findings demonstrate that seed-priming with Si is an efficient management technique thatcan be used to alleviate deleterious effects of salt-stressful on germination of lettuce seeds and enhance salt tolerance of seedlings due the increased activity of ROS- scavenging enzymes.

https://doi.org/10.1590/1678-4499.20190360 1094 downloads

11.

Snake venoms and purified toxins as biotechnological tools to control Ralstonia solanacearum
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Alves, Rita de Cássia

; Vieira Júnior, José Roberto

; Freire, Tamiris Chaves

; Fonseca, Aline Souza da

; Sangi, Simone Carvalho

; Barbieri, Fábio da Silva

; Rocha, Rodrigo Barros

; Brito, Luciana Gatto

; Pereira, Soraya dos Santos

; Luiz, Marcos Barros

; Freire, Francisco das Chagas Oliveira

; Fernandes, Carla Freire Celedonio

; Soares, Andreimar Martins

; Fernandes, Cléberson de Freitas

Pesquisa Agropecuária Brasileira

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2020, Volume 55 elocation e01756

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

Resumo: O objetivo deste trabalho foi avaliar a atividade antibacteriana in vitro de venenos e toxinas purificadas de serpentes sobre a bactéria fitopatogênica Ralstonia solanacearum. As avaliações foram realizadas em 17 venenos brutos (13 de Bothrops, 3 de Crotalus e 1 de Lachesis) e sete toxinas (1 de Bothrops e 6 de Crotalus). A atividade antibacteriana foi avaliada em meio MB1 que continha os tratamentos solubilizados (1 μL mL-1). Utilizou-se o total de 100 μL de suspensão bacteriana (8,4 x 109 UFC mL-1). Após incubação a 28°C, avaliou-se o número de colônias bacterianas às 24, 48 e 72 horas após a inoculação. O gel SDS-PAGE a 15% foi usado para analisar o perfil proteico das amostras, tendo-se utilizado 5 μg de proteína no ensaio. Além disso, os valores de concentração inibitória mínima (CIM) e concentração letal (CL50) foram determinados pelo método Probit. Os venenos e as toxinas foram capazes de reduzir mais de 90% do crescimento de R. solanacearum. Esses resultados foram ou equivalentes aos do controle positivo cloranfenicol ou até melhores. Enquanto os valores de CIM variaram de 4,0 a 271,5 μg mL-1, a CL50 variou de 28,5 μg mL-1 a 4,38 mg mL-1. Dez venenos brutos (7 de Bothrops e 3 de Crotalus) e duas toxinas (giroxina e crotamina) são abordagens promissoras para o controle da bactéria fitopatogênica R. solanacearum.

Abstract: The objective of this work was to evaluate the in vitro antibacterial activity of snake venoms and purified toxins on the phytopathogenic bacterium Ralstonia solanacearum. The evaluations were performed with 17 crude venoms (13 from Bothrops, 3 from Crotalus, and 1 from Lachesis) and seven toxins (1 from Bothrops and 6 from Crotalus). Antibacterial activity was assessed in MB1 medium containing solubilized treatments (1 μL mL-1). A total of 100 μL bacterial suspension (8.4 x 109 CFU mL-1) was used. After incubation at 28°C, the number of bacterial colonies at 24, 48, and 72 hours after inoculation was evaluated. SDS-PAGE gel at 15% was used to analyze the protein patterns of the samples, using 5 μg protein of each sample in the assay. Furthermore, the minimum inhibitory concentration (MIC) and lethal concentration (LC50) values were determined by the Probit method. Venoms and toxins were able to reduce more than 90% of R. solanacearum growth. These results were either equivalent to those of the positive control chloramphenicol or even better. While MIC values ranged from 4.0 to 271.5 μg mL-1, LC50 ranged from 28.5 μg mL-1 to 4.38 mg mL-1. Ten crude venoms (7 from Bothrops and 3 from Crotalus) and two purified toxins (gyroxin and crotamine) are promising approaches to control the phytopathogenic bacterium R. solanacearum.

https://doi.org/10.1590/s1678-3921.pab2020.v55.01756 478 downloads

12.

Pretreatment of forage legumes under moderate salinity with exogenous salicylic acid or spermidine
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Pizolato Neto, Antonio

; Alves, Rita de Cássia

; Camargos, Ayza Eugênio Viana

; Gratão, Priscila Lupino

; Carregari, Sônia Maria Raymundo

; Zingaretti, Sonia Marli

; Santos, Durvalina Maria Mathias Dos

Acta Scientiarum. Agronomy

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2020, Volume 42 elocation e42809

Resumo: En | Texto: En | PDF: En

ABSTRACT. The present study aims to determine whether exogenous salicylic acid (SA) or spermidine (Spd) has any protective effect against salt stress. Seeds were subjected to 0, 20, 40, and 60 mM NaCl with or without salicylic acid or spermidine (0.5 mM) for 10 days. The evaluated variables were germination rate, shoot and root dry masses, glycine betaine content, lipid peroxidation, and the activities of superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxidase (APX). The data were subjected to Tukey’s test (p ≤ 0.05). There was a growth increase, especially in plant shoots. The reduction in lipid peroxidation, as indicated by lower malondialdehyde (MDA) levels, can be explained by an increase in antioxidant activity when SA and Spd were added. When compared to CAT and APX, SOD was the least responsive enzyme to the addition of both SA and Spd in salt-stressed plants. SA and Spd partially reduced the effects of moderate salt stress in both plant species; however, Spd addition had better results than SA in terms of suppressing oxidative stress. Lablab plants were more vigorous than pigeonpea plants.

https://doi.org/10.4025/actasciagron.v42i1.42809 394 downloads

13.

Bell pepper production under saline stress and fertigation with different K+/Ca2+ ratios in a protected environment
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Silva, Ricardo Carlos Pereira da

; Oliveira, Francisco de Assis de

; Oliveira, Ademar Pereira de

; Medeiros, José Francismar de

; Alves, Rita de Cássia

; Paiva, Francisco Italo Gomes

Acta Scientiarum. Agronomy

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2020, Volume 42 elocation e42498

Resumo: En | Texto: En | PDF: En

ABSTRACT. Bell peppers are sensitive to salinity; therefore, it is necessary to find alternatives to reduce saline stress. An experiment was conducted in a greenhouse at Federal Rural University of the Semi-Arid Region, in Mossoró, Rio Grande do Norte State, Brazil, to evaluate the effects of salinity and K+/Ca2+ ratios on bell pepper production. The experimental design consisted of randomized blocks in a 5 x 4 factorial scheme with four replicates, corresponding to five K+/Ca2+ ratios (F1 = 3.3/1, F2 = 2.8/1, F3 = 2.2/1, F4 = 1.8/1, and F5 = 1.5/1) and four salinity levels in the nutrient solution using NaCl (1.75, 3.25, 4.75, and 6.25 dS m-1). The following parameters were evaluated: the number of fruits (total, marketable and unmarketable), the mean weight of fruits (marketable and unmarketable), fruit production (total, marketable and percentage of marketable fruits) and a salinity tolerance index. Generally, nutrient solution enrichment with K+ or Ca2+ did not cause significant increments in bell pepper yield. The fertigation treatments F2, F3, and F5 led to a higher bell pepper tolerance to salinity, allowing waters with higher salt concentrations to be used without causing a reduction in the yield.

https://doi.org/10.4025/actasciagron.v42i1.42498 439 downloads

14.

Geração de Curvas IDFs para Cenários Projetados na Cidade de Porto Alegre/RS
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Weschenfelder, Adriana Burin

; Klering, Eliana Veleda

; Alves, Rita de Cássia Marques

; Pinto, Éber José de Andrade

Revista Brasileira de Meteorologia

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Jun 2019, Volume 34 Nº 2 Páginas 201 - 216

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

Resumo O aumento na frequência de eventos extremos associados às alterações climáticas podem modificar as curvas IDFs existentes. A metodologia para definição de curvas IDF considerando cenários futuros consistiu em calibrar o modelo estocástico para gerar séries sintéticas diárias de precipitação com base em dados observados do município de Porto Alegre e gerar séries sintéticas perturbadas a partir de seis MCGs, três cenários A1B, A2 e B1 para os períodos de 2046 a 2065 e 2080 a 2099. Na elaboração das IDFs foram desagregados os dados sintéticos diários, tanto para a linha de base (1974 a 2014), quanto para os cenários projetados, utilizando as relações de desagregação da IDF atual de Porto Alegre. Os resultados obtidos após a calibração indicaram ser o modelo estocástico utilizado eficiente na representação dos dados local. Os resultados encontrados para as projeções no primeiro período indicam que as simulações dos modelos são razoavelmente aplicáveis, sugerindo a utilização da curva IDF atual com uma majoração de 10 a 20% no dimensionamento de obras hidráulicas. Já para o segundo período de projeção em função da grande variabilidade nos resultados encontrados para os diferentes modelos não há confiança na aplicabilidade destas projeções.

Abstract The increase in the frequency of extreme events associated with climate change can modify the existing IDF curves. The methodology for defining IDF curves considering future scenarios was to calibrate the stochastic model to generate daily synthetic precipitation series based on observed data from the city of Porto Alegre and to generate perturbed synthetic series from six MCGs, three scenarios A1B, A2 and B1 for the periods 2046 to 2065 and 2080 to 2099. In the elaboration of the IDFs, the daily synthetic data were disaggregated, both for the baseline (1974 to 2014) and for the projected scenarios, using the disaggregation relationships of the current IDF of Porto Alegre. The results obtained after the calibration indicated that the stochastic model used was efficient in the local data representation. The results for the projections in the first period indicate that the simulations of the models are reasonably applicable, suggesting the use of the current IDF curve with a 10 to 20% increase in the design of hydraulic works. Already in the second projection period due to the great variability in the results found for the different models, there is no confidence in the applicability of these projections

https://doi.org/10.1590/0102-77863340026 1466 downloads

15.

Hepatitis C Virus Core Antigen Test: an HCV RNA screening alternative in end-stage renal disease and hemodialysis?
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Alves, Rita V

; Gonçalves, Hernâni

; Gonçalves, Maria L

; Lopes, Karina

; Sofia, Flora

; Lobos, Ana V

Portuguese Journal of Nephrology & Hypertension

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Jun 2019, Volume 33 Nº 2 Páginas 87 - 90

Resumo: En | Texto: En | PDF: En

Introduction and Aim:Hepatitis C virus screening using anti-HCV antibody is recommended for end-stage renal disease and chronic hemodialysis patients. Since the anti-HCV antibody test is not able to differentiate a concurrent infection from a resolved past one, a more reliable and cost-effective screening test is needed. Materials and Methods: Prospective study of end-stage renal disease and chronic hemodialysis patients from a hospital unit who were screened by anti-HCV antibody and total ARCHITECT® HCV antigen assay. Results: Cohen's κ test was run to determine if there was agreement between HCV Ribonucleic Acid and HCV core antigen assays on 7 individuals with positive anti-HCV antibody. The two tests had a perfect measurement agreement. Conclusions: HCV core antigen testing could be used as a sensitive method for HCV infection screening in this group of patients based on its accuracy, simplicity and low expense

https://doi.org/10.32932/pjnh.2019.07.019 431 downloads

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